Use our product documents resource to find product manuals, help guides, coq, msds and more. The guava easycyte ht system users guide provides detailed information on. Guava is the combination of numerous contributors, with a team that ensures the apis are consistent, allowing it to continuously evolve. Guava easycyte, guava easycyte plus system computer for acquisition purposes. The guava instruments are based on microcapillary flow. Load the ion chef system and clean the ion chef instrument topics reorganized for ease of use. The ion 540 kit ot2 is compatible with torrent suite software v5. Ion sphere particles quality assessment for the ion proton and ion s5 systems using the guava easycyte 5 benchtop flow cytometer publication number man0007496 revision b. When using third party programs to analyze exported data, please be sure to read the disclaimers in a previous section of this document. The manual loader holds two separate tubes, allowing the user to progress quickly from one sample to another, or to wash between samples following an.
Inside the guava easycyte 8ht and easycyte 8 systems see page 2 for specs for easycyte 5, 6, 62l, 5ht, 6ht and 6ht2l visits. Millipore guava ht with plate handler protocol for multiplex assays this protocol contains instruction for setting up a millipore guava ht system. Now you can screen plates of cells and compounds quickly and easily, without the hassle of manipulating multiple tub,biological,biology supply,biology supplies,biology product. Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. These simple fluidics enable a selfaligning flow cell that can be removed for cleaning by the user. Spectrometers shared equipment authority rice university. Rapid, precise, and accurate counts of symbiodinium cells. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less. Guava software modules show all relevant data and results immediately. References to isp quality control user guides updated to the ion sphere quality control kit user guide pub.
If you need technical support, you can contact us 24 hours a day by email, call 18777852323, or visit our customer center for more local help. Please refer to the appropriate package insert for specific instructions and limitations. Ion sphere particles quality assessment user bulletin 7. A guava easycyte ht 2laser flow cytometer millipore was used with excitation by the blue 488nm laser and the gain controls at their default settings yellow and green fluorescence or set to 9. Determinations of algal numbers using the guava flow cytometer. If you created a worklist already, click start worklist. G112936 guava technologies guava easycyte plus w2 flow cells. Guava easycyte from guava technologies,the guava easycyte system is the first and only blue laser based multiparameter cytometer with builtin 96well microplate sampling with tube options. Be sure to update your torrent server to the latest available version of torrent suite software before using this kit. Guava is an open source, javabased library and contains many core libraries. Guava easycyte mini from guava technologies biocompare. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays in a highly userfriendly fashion and can handle 96well plates and up to 10 tubes in a single load. Setup procedure for guava easycyte with two lasers. The user of this ebook is prohibited to reuse, retain, copy, distribute or republish.
For fully automated sample loading from single tubes or a 96well plate, we offer the easycyte ht high throughput systems, with four available highpower lasers. Guava easycyte system users guide guava pulp and guava leather. The best resource is to monitor the users group, blogs, and presentations. Clarification made to dilute barcoded library pools, if used. Setup procedure for guava easycyte with two lasers important. Has anyone experience with millipores guava flow cytometer.
Figure 1a illustrates the overall standard cure conformity among. Flow cells for newer guava easycyte systems now include minstac tubing. Use the following metrics together to evaluate a sample, but not as. The guava easycyte 5 flow cytometer offers absolute cell counts and five parameters 3 fluorescent colors, 2 light scatter, from an industry standard blue laser. The user of this ebook is prohibited to reuse, retain, copy, distribute or republish any contents or a part of contents of this ebook in any manner without written consent of the publisher.
Blue 488 nm 150 mw and green 532 nm 100mw with 8 fluorescent detection channels, forward and side scatter. This guide is intended for the guava easycyte 5, 62l, 8, and 12 instruments. The easycyte mini is a benchtop flow cytometry system by guava. The millipore guava easycyte ht flow cytometer is a flexible, compact and reliable high throughput benchtop system. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays in a highly user friendly fashion and can handle 96well plates and up to 10 tubes in a single load.
This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays. The guava easycyte ht doesnt require sheathing fluid, so you can. Innovative guava microcapillary technology in the guava muse cell analyzer provides miniaturized systems for quick cell counts, viability, and basic cell health analyses, or step up to the guava easycyte system, a versatile benchtop platform for additional, multidimensional cell health assessments. This pc controlled cell analyzer utilizes microsyringe fluidics technology in conjunction with a 488 nm blue diode laser, forward scatter, and photomultipliers with three filters yellow583 nm, red680 nm, and green525 nm for conducting fluorescence detection. Manual adjustment for gain on the laser exciting the reporter molecule was not possible for instruments a and f. Guava easycyte from guava technologies biomedicine. The aim of this study was to adapt the immunohistochemical. Dweck fls frsc frsh dweck data introduction in the next of our series on far eastern plant we look at guava or psidium guajava in folk medicine, extracts of roots, bark, and leaves are used to treat gastroenteritis. Please refer to the appropriate package insert for specific instructions and. Guava easycyte 5ht base system from luminex selectscience.
I have a demonstration in a couple of days and im interested in researchers experiences. Inside the guava easycyte 12ht and guava easycyte 12 systems see page 7 for specifications. As a result, guava easycyte flow cytometers are uniquely amenable to ondemand use in the laboratory environment and have helped many. The instrument accommodates 96well plates and up to 10 tubes in a single load. Ion pgm hi q view ot2 kit thermo fisher scientific. An easy to use, simple, compact, robust instrument for your cell analysis needs sophisticated cell analysis doesnt have to be complicated or costly.
Millipore guava easycyte ht flow cytometer system youtube. They use novel microcapillary technology and they are compact, user friendly, and powerful enough emd millipore afs analyzer feed systems find emd millipore afs analyzer feed systems forums, questions, troubleshooting, discussions at. How it works the guava easycyte systems use patented, microcapillary, laserbased technology capable of detecting mammalian and microbial cells and beads. The kit contains easycheck beads and the easycheck diluent. If the correct dilution factor was entered in the guava express pro software, you can determine the total number of recovered isps as follows. Guava easycyte system users guide free ebook download as pdf file. Millipore guava easycyte ht flow cytometer system available from the lab world group. The guava easycyte 5ht system provides absolute cell counts and offers other highdemand features, such as five parameters 3 fluorescent colors, 2 light scatter and has a blue laser for access to commonly used fluorescent dyes. These simple fluidics enable a selfaligning flow cell that can be removed for cleaning by. Millipore guava ht with plate handler protocol for multiplex. Setup procedure for guava easycyte with a single laser important note. Consult the guava easycyte system users guide, guava easycyte ht system users guide or guava easycyte plus system users guide for more details. Has anyone experience with the easycyte 5 flow cytometer from millipore.
Its single sample loader offers reliable tube sampling. With the incorporation of the microsyringe fluidics, the read more. Guava easycheck software module issues addressed backflush for easycyte systems was not working in easycheck 2. The m1 cellml shows the total number of recovered isps. Jun, 2007 guava easycyte mini is a microsyringebased flow cytometer equipped with a 488 nm blue laser, forward scatter detection and three channels for fluorescence detection. Ion pgm hi q view chef kits thermo fisher scientific. Interested users must schedule an appointment for training with the cytometry core to discuss experimental design and expected outcomes. Consult the guava easycyte system user s guide, guava easycyte ht system user s guide or guava easycyte plus system user s guide for more details. The university of sydneys major institute for medical research seeks to answer questions about the human body in health and in illness. Use manual viacount analysis under these circumstances. Rapid, effective and userfriendly immunophenotyping of.
The guava easycyte mini was introduced to the cytometry core facility in january of 2010 through a partnership agreement with dr. Ion sphere particles quality assessment for the ion proton. The muse cell analyzer packs 3parameter analysis into a compact. Guava easycyte system users guide cytotoxicity apoptosis. Setup procedure for guava easycyte with a single laser. In addition, it is equipped with a highthroughput sampler able to automatically measure samples directly from a 96well plate or via micro. They use novel microcapillary technology and they are compact, userfriendly, and powerful enough emd millipore afs analyzer feed systems find emd millipore afs analyzer feed systems forums, questions, troubleshooting, discussions at. Running the easycheck procedure for daily quality control qc and maintenance ensures you are.
Guava easycyte ht bg system is a flow cytometer for performing flow cytometry experiments. Find msds or sds, a coa, data sheets and more information. Mitosox red is excitable by a 488nm laser and fluoresces maximally at 580 nm yellow fluorescence on the guava easycyte 8ht. Summary of new features, changes and enhancements since guavasoft software v3. No laser alignment or sheath fluid required uses smaller sample volume and generates less. Guava easycyte ht bg flow cytometer shared equipment. We strive to update the contents of our website and tutorials as timely and as precisely as. Guava easycyte flow cytometers from luminex corporation. Dweck fls frsc frsh dweck data introduction in the next of our series on far eastern plant we look at guava or psidium guajava in folk medicine, extracts of roots, bark, and leaves are used to treat gastroenteritis, vomiting, diarrhoea, dysentery, wounds, ulcers, toothache, coughs, sore throat. Follow the software instructions if prompted to put required tubessolutions for. Optical layout for the easycyte 12 threelaser system. Guava easycyte flow cytometers from luminex corporation description youll quickly find that guava easycyte benchtop microcapillary flow cytometers are simpler and cheaper to operate and maintain than traditional sheath fluidbased instruments enabling ondemand use in any lab. Comparison and evaluation of seven different bench. The guava easycyte plus guava technologies, hayward, ca, us is the first, personal, benchtop flow cytometer designed to address these limitations.